protein lysate (Jena Bioscience)
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Jena Bioscience
protein lysate
Protein Lysate, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy5+5+azide/Cy5%2E5-Azide/pmc13096800-47-10-3
Average 94 stars, based on 8 article reviews
Protein Lysate, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cy5+5+azide/Cy5%2E5-Azide/pmc13096800-47-10-3
Average 94 stars, based on 8 article reviews
protein lysate - by Bioz Stars,
2026-09
94/100 stars
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Imaging:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Isolation:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Centrifugation:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Membrane:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Fluorescence:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Immunoprecipitation:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Western Blot:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Expressing:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Comparison:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Concentration Assay:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, SDS Page:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Functional Assay:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, In Vivo:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Pulse Chase:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Incubation:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Irradiation:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Conjugation Assay:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Stable Transfection:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Transfection:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Negative Control:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, Affinity Purification:Article Title: Rapid photo-crosslinking in living cells reveals protein–nucleic acid dynamics on a timescale of minutes Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5-azide ( Article Title: Rapid photo-crosslinking in living cells reveals protein-nucleic acid dynamics on a timescale of minutes. Article Snippet: protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer. .. Fluorescent dye Cy5.5azide ( Article Title: Enhanced photo-crosslinking in living cells with high-intensity longwave ultraviolet light Article Snippet: The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.The protein content was determined by BCA assay and protein concentrations adjusted to 1 mg/ml with denaturing lysis buffer.. Fluorescent dye Cy5.5-azide ( Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample. Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl1H-1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Cell Labeling with 15-YNE Is Useful for Tracking Protein Palmitoylation and Metabolic Lipid Flux in the Same Sample Article Snippet: the click reaction, 50 µg and 400 µg of protein were used with either Cy5.5-azide or biotin-azide, respectively. .. The click reaction was initiated by the addition of CuSO 4 (Sigma Aldrich, Merck, Darmstadt, Germany), Tris[(1-benzyl-1 H -1,2,3-triazol-4-yl)methyl]amine (TBTA, Sigma-Aldrich, Merck, Germany), Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: Beads were washed 4 times into 50 mM Tris.HCl pH 8.0 and Article Title: Photo-affinity labelling and biochemical analyses identify the target of trypanocidal simplified natural product analogues Article Snippet: probe was conjugated to alkyne-tagged protein as previously reported [ ]. .. Briefly, |